Monkey PD-1[Biotinylated]:PD-L1 Inhibitor Screening Assay Kit

• Background:

  PD-L1 and PD-L2 binding to PD-1, a receptor expressed on T-cells, negatively regulates immune responses. PD-1 ligands PD-L1 and PD-L2 are found on the surface of many cancer cells, and their interaction with receptor PD-1 inhibits T cell activity and allows cancer cells to escape immune surveillance. This pathway is also involved in regulating autoimmune responses. Therefore, these proteins (termed immune checkpoints) are promising therapeutic targets for many types of cancer as well as multiple sclerosis, arthritis, lupus, and type I diabetes. Checkpoint inhibitors have remarkable efficacy in a wide range of cancer types and have revolutionized cancer treatment. PD-1 inhibitors nivolumab, pembrolizumab, cemiplimab and PD-L1 inhibitors atezolizumab, avelumab, and durvalumab are all FDA-approved drugs for immuno-therapy.

• Description:

  The Monkey PD1[Biotinylated]: PD-L1 Inhibitor Screening Assay Kit is a chemiluminescence assay designed for screening and profiling molecules that block the binding of PD-1 (programmed  cell death protein 1) to PD-L1 (programmed cell death 1 ligand 1) . This assay kit comes in a convenient 96-well format, with enough purified monkey biotin-labeled PD-1 (amino acids 25-167), monkey PD-L1 (amino acids 25-167), assay buffers and detection reagents for 100 enzyme reactions.The key to this kit is the high affinity of biotin-labeled PD-1 for streptavidin-HRP. First, a 96-well plate is coated with PD-L1, followed by incubation with PD-1. Finally, the plate is treated with streptavidin-HRP followed by addition of HRP substrate. The chemiluminescence signal can be detected with a microplate reader capable of reading chemiluminescence. The signal generated is proportional to the binding between PD-1 and PD-L1.

• Applications:

  Screening small molecule inhibitors that block monkey PD-1 binding to monkey PD-L1 for drug discovery and high throughput screening (HTS) applications.

• Format:

  Catalog #NameAmountStorage71153PD-L1 (Monkey), Fc fusion (Human IgG1) *10 µg-80°C71235PD-1 (Monkey), Fc fusion (Human IgG1), Biotin-Labeled *5 µg-80°C793113x Immuno Buffer 150 ml-20°C79728Blocking Buffer 250 ml+4°C79742Streptavidin-HRP10 µl+4°C79670ELISA ECL Substrate A (translucent bottle) 6 mlRoom TempELISA ECL Substrate B (brown bottle) 6 mlRoom Temp7969996-well white plate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

• Notes:

  Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]

• Contraindications:

  This kit is compatible with up to 1% final DMSO concentration.