PD-1:PD-L1[Biotinylated] Inhibitor Screening Colorimetric Assay Kit

• Description:

  Cell signaling through the PD-1 receptor upon binding the PD-L1 ligand attenuates immune responses and is exploited by both tumors and viruses. The PD-1:PDL1[Biotinylated] Inhibitor Screening Colorimetric Assay Kit is designed for screening inhibitors of PD-L1 ligand binding to PD-1 receptor. The key to this kit is the high affinity of biotin-labeled PD-L1 for streptavidin-HRP. Only a few simple steps on a microtiter plate are required for the assay. First, PD-1 is coated on a 96-well plate. Next, PD-L1 is incubated with PD-1 on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can then be measured using a UV/Vis spectrophotometer microplate reader.Need us to run inhibitor screens or profile your compounds against PD-1:PD-L1[Biotinylated]? Check out ourImmunotherapy Biochemical Screening Services.This product has beencited 2 times.

• UniProt:

  PD-1: Q15116; PD-L1: Q9NZQ7

• Applications:

  This kit is useful for screening for inhibitors of PD-1 binding to PD-L1.

• Format:

  Catalog#ComponentAmountStorage71105PD-L1, Biotin-labeled (Human) 5 µg-80°CAvoid freeze/thaw cylces71106PD-1 (Human) 10 µg-80°C79742Streptavidin-HRP10 µl+4°C793113x Immuno Buffer 150 ml-20°C79728Blocking Buffer 250 ml+4°C79651Colorimetric HRP substrate10 ml+4°C79964Transparent 96-well microplate1+4°C

• Shipping Conditions:

  -80°C (Dry Ice)

• Storage Conditions:

  Stable at least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.