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CHK2 Kinase Assay Kit

CAT: 0421-78421Size: 96 ReactionsDry Ice: YesHazardous: No
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CAT#:0421-78421Size:96 Reactions
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Background
CHK2 (checkpoint kinase 2, also known as CHEK2) is an evolutionarily conserved serine/threonine protein kinase that operates as a cell cycle checkpoint in response to DNA damage. Thus, it is rapidly phosphorylated and activated upon DNA damage. It is known to phosphorylate and inactivate phosphatase Cdc25C, thereby preventing entry into mitosis. CHK2 also stabilizes tumor suppressor TP53 and is a key player in the ATM DNA damage response and repair pathway. CHK2 mutations are observed in Li-Fraumeni syndrome, an inherited predisposition to cancer. Mutations in CHK2 are also linked to various spontaneous types of cancer.
Description
The CHK2 Kinase Assay Kit is designed to measure CHK2 kinase activity for screening and profiling applications using ADP-Glo® as a detection reagent.
UniProt
O96017
Applications
Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications.
Format
Catalog #NameAmountStorage40039CHK2*2.5 µg-80°C793345x Kinase assay buffer1.5 ml-20°C79686ATP (500 µM) 100 µl-20°C78419CHKtide (1 mg/ml) 500 µl-20°C7969696-well plate, white1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.
Shipping Conditions
-80°C
Storage Conditions
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Notes
Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]
Contraindications
The final concentration of DMSO in the assay should not exceed 1%.

UniProtKB · O96017

Serine/threonine-protein kinase Chk2

CHK2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O96017
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CHEK2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.7.11.1
Processing
—
Secondary accessions
A8K3Y9, B7ZBF3, B7ZBF4, B7ZBF5, Q6QA03, Q6QA04, Q6QA05, Q6QA06, Q6QA07, Q6QA08, Q6QA10, Q6QA11, Q6QA12, Q6QA13, Q9HBS5, Q9HCQ8, Q9UGF0, Q9UGF1
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisCell cycleCell divisionDNA damageDNA repairHost-virus interactionMitosisTranscriptionTranscription regulation

Ligand

ATP-bindingMagnesiumMetal-bindingNucleotide-binding

Disease

Disease variantLi-Fraumeni syndromeTumor suppressor

Molecular function

KinaseSerine/threonine-protein kinaseTransferase

Cellular component

Nucleus

PTM

PhosphoproteinUbl conjugation

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Alternative Products

CatalogName
abx133083-01Serine/threonine-Protein Kinase Chk2 (CHK2) Antibody
310710-01Chk2 (CHEK2, CDS1, CHK2, RAD53, Serine/threonine-protein kinase Chk2, CHK2 checkpoint homolog, Cds1 homolog, Hucds1, hCds1, Checkpoint kinase 2)
C4201-05HCHK2 (Serine/Threonine-protein Kinase Chk2, Cds1, CHEK2, RAD53)
350579Chk2, CT (CHEK2, Serine/threonine-protein kinase Chk2, Checkpoint kinase 2, RAD53)
298650Chk2 (Checkpoint kinase 2) discontinued
221690-01Chk2 (Serine/threonine-Protein Kinase Chk2, CHK2 Checkpoint Homolog, Cds1 Homolog, Hucds1, hCds1, Checkpoint Kinase 2, CHEK2, CDS1, CHK2, RAD53) discontinued
201-12-8720Human Serine/threonine-protein kinase CHK2 (CHK2/CHEK2) ELISA Kit
C4200-90NCHEK2 (Serine/Threonine-protein Kinase Chk2, Cds1, CHK2, RAD53) discontinued
C4201-05FCHK2 (Serine/Threonine-protein Kinase Chk2, Cds1, CHEK2, RAD53) discontinued
KBH15946GENLISA Human Serine / threonine-Protein Kinase CHK2 (CHK2 / CHEK2) ELISA

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