Spike Trimer (S1+S2) (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2) : ACE2 Inhibitor Screening Colorimetric Assay Kit

• Background:

  The COVID-19 pandemic is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) . The Spike glycoprotein is expressed on the surface of the virus as a trimer. Each Spike protein consists of two subunits, S1 and S2, and the S1 subunit contains the receptor binding domain (RBD) which recognizes and attaches to the ACE2 (angiotensin-converting enzyme 2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. SARS-CoV-2 Variant B.1.1.529 BA.1, also known as Omicron variant, was originally discovered in South Africa and became a global variant of concern. This variant contains several mutations that increase infectivity and transmissibility. Drugs targeting the interaction between SARS-CoV-2 Spike protein and human ACE2 may offer some protection against viral infection.

• Description:

  The Spike Trimer (S1+S2) (B.1.1.529 BA.1, Omicron Variant) (SARS-CoV-2) : ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors or neutralizing antibodies of the interaction between the SARS-CoV-2 Omicron variant Spike Trimer and human ACE2 (angiotensin-converting enzyme 2) . This kit comes in a convenient 96-well format, with Biotinylated-ACE2 (amino acids 18-740), purified Spike Trimer protein (B.1.1.529 BA.1, Omicron Variant) (amino acids 16-1213), Streptavidin-HRP, and assay buffers for 100 reactions. The SARS-CoV-2 Spike Trimer protein, included in the kit, provides a biologically relevant model for the investigation of SARS-CoV-2/ACE2 interactionFigure 1. Assay Kit schematic.First, SARS-CoV-2 Spike Trimer (S1+S2) (B.1.1.529 BA.1, Omicron Variant) is coated on a 96-well plate overnight. After washing and blocking, the protein is pre-incubated with an inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP. After a final wash, Colorimetric HRP substrate is added to produce color that can be measured using a UV/Vis spectrophotometer microplate reader. The colorimetric signal is proportional to the efficacy of the binding of Spike Trimer (S1+S2) (B.1.1.529 BA.1, Omicron Variant) to ACE2.Need us to run an inhibitor screening or profiling? Check out ourCoronavirus Biochemical Screening Services.

• Applications:

  Screening inhibitors of ACE2 binding to SARS-CoV-2 Spike Trimer (S1+S2) (B.1.1.529 BA.1, Omicron Variant) .

• Format:

  Catalog #NameAmountStorage101343Spike Trimer (S1+S2) (B.1.1.529 BA.1, Omicron Variant), His-Tag (SARS-CoV-2) *20 µg-80°C100665ACE2, His-Avi-Tag, Biotin labeled*2 x 5 µg-80°C793113x Immuno Buffer 150 ml-20°C79728Blocking Buffer 250 ml+4°C79742Streptavidin-HRP10 µl+4°C79651Colorimetric HRP substrate10 ml+4°C79964Transparent 96-well microplate1Room Temp*The initial concentration of the proteins is lot-specific and will be indicated on the tube containing the protein.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

• Notes:

  Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]

• Contraindications:

  This assay kit is compatible with up to 1% final DMSO concentration.