Spike Trimer (S1+S2) (P.1; Gamma Variant) (SARS-CoV-2) : ACE2 Inhibitor Screening Colorimetric Assay Kit

• Background:

  The COVID-19 pandemic is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) . The Spike glycoprotein is expressed on the surface of the virus as a trimer. Each Spike protein consists of two subunits, S1 and S2, and the S1 subunit contains the receptor binding domain (RBD) which recognizes and attaches to the ACE2 receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. The P.1 variant of the SARS-CoV-2 Spike protein (also known as Gamma variant) was originally discovered in Brazil and contains 11 mutations (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y, T1027I) . Drugs targeting the interaction between SARS-CoV-2 Spike and human ACE2 may offer some protection against viral infection.  This kit includes theSpike Trimer (S1+S2) (P.1; Gamma Variant) (SARS-CoV-2) (BPS Bioscience #100989) in its native trimeric conformation to provide a physiologically relevant screen for inhibitors of the Spike S1:ACE2 interaction.

• Description:

  The Spike Trimer (S1+S2) (P.1; Gamma Variant) (SARS-CoV-2) : ACE2 Inhibitor Screening Colorimetric Assay Kit is designed for screening and profiling inhibitors or neutralizing antibodies of  the interaction between SARS-CoV-2 Spike and human ACE2. The key to this kit is that the Spike Trimer (S1+S2) (P.1 Variant) (SARS-CoV-2) protein provides a biologically relevant model for the investigation of SARS-CoV-2/host cell interaction.The assay requires only a few steps. First, Spike Trimer (S1+S2) (Gamma Variant) (SARS-CoV-2) is coated on a 96-well plate overnight. After blocking, the protein is pre-incubated with the inhibitor or neutralizing antibody. Upon subsequent incubation with Biotin-ACE2, the plate is treated with Streptavidin-HRP followed by addition of a colorimetric HRP substrate to produce color, which can be quenched and measured using a UV/Vis microplate reader.

• Applications:

  This kit is useful for screening inhibitors of ACE2 binding toSpike Trimer (S1+S2) (P.1 Variant) (SARS-CoV-2)

• Format:

  Catalog #NameAmountStorage100989Spike Trimer (S1+S2) (P.1 Variant), His-Tag (SARS-CoV-2) *5 µg-80°C100665ACE2, His-Avi-Tag, Biotin labeled *2 x 5 µg-80°C793113x Immuno Buffer 150 ml-20°C79728Blocking Buffer 250 ml+4°C79742Streptavidin-HRP5 µl+4°C79651Colorimetric HRP substrate10 ml+4°CTransparent 96-well microplate1Room Temp*The initial concentration of both ACE2 and Spike Trimer is lot-specific and will be indicated on the tube containing the protein.

• Shipping Conditions:

  -80°C

• Storage Conditions:

  This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.

• Notes:

  Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]

• Contraindications:

  DMSO concentration in the final reaction should be ≤1%.