Products for Research Use Only
RegisterLogin

PCSK9[Biotinylated]-LDLR Binding Assay Kit

CAT: 0421-72002Size: 96 ReactionsDry Ice: YesHazardous: No
Product image 1
1 / 1
CAT#:0421-72002Size:96 Reactions
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
PCSK9 regulates circulating cholesterol homeostasis by binding to the ectodomain of liver low-density lipid receptors and promoting their degradation. PCSK9 binds to the low density lipoprotein receptor (LDLR), the very low density lipoprotein receptor (VLDLR), the apolipoprotein E receptor (LRP1/APOER), and the apolipoprotein receptor 2 (LRP8/APOER2) .
Description
The PCSK9[Biotinylated]-LDLR Binding Assay Kit is designed for screening and profiling purposes. PCSK9 is known to function as a negative regulator of hepatic low-density lipoprotein receptors (LDLRs) by binding to the LDLR ectodomain. The key to this kit is the high sensitivity of detection of biotin-labeled PCSK9 by streptavidin-HRP. Only a few steps are required for the assay. First, LDLR ectodomain is coated on a 96-well plate. Next, PCSK9 is incubated with LDLR on the plate. Finally, the plate is treated with streptavidin-HRP followed by addition of an HRP substrate to produce chemiluminescence, measured using a chemiluminescence reader.*NOTE: As of November 2022, this protocol has been re-optimized for performance. Previous versions of this kit are available upon request.Need us to run inhibitor screens or profile your compounds against PCSK9-LDLR? Check out ourPCSK9 Screening Services.This product has beencited 7 times.
UniProt
Q8NBP7
Applications
Screen or titer small molecules and antibodies that inhibit the binding of PCSK9 to LDLR ectodomain.
Format
Catalog #NameAmountStorage71304PCSK9[Biotinylated]*10 µg-80°CAvoid multiple freeze/ thaw cycles71205LDLR*10 µg-80°C79742Streptavidin-HRP10 µl+4°C332983x PL-01 Assay Buffer50 ml-20°C79728Blocking buffer 250 ml+4°C79670ELISA ECL Substrate A (translucent bottle) 6 mlRoom TempELISA ECL Substrate B (brown bottle) 6 mlRoom Temp79699White 96-well microplate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.
Shipping Conditions
-80°C
Storage Conditions
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Contraindications
PCSK9[Biotinylated]-LDLR Binding Assay Kit is compatible with up to 1% final DMSO concentration. We recommend preparing the inhibitor in no higher than 10% DMSO solution in buffer and using 5 µl per well.

UniProtKB · Q8NBP7

Proprotein convertase subtilisin/kexin type 9

PCSK9_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q8NBP7
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PCSK9
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.21.-
Processing
Precursor
Secondary accessions
A8T640, C0JYY9, Q5PSM5, Q5SZQ2
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisCholesterol metabolismLipid metabolismSteroid metabolismSterol metabolism

PTM

Autocatalytic cleavageDisulfide bondGlycoproteinPhosphoproteinSulfationZymogen

Ligand

Calcium

Cellular component

CytoplasmEndoplasmic reticulumEndosomeGolgi apparatusLysosomeSecreted

Disease

Disease variant

Molecular function

HydrolaseProteaseSerine protease

Domain

Signal

Related Products

CatalogName
78866PCSK9 (D374Y) [Biotinylated]-LDLR Binding Assay Kit
78326-1PCSK9 (D374T) [Biotinylated]-LDLR Binding Assay Kit
78326-2PCSK9 (D374T) [Biotinylated]-LDLR Binding Assay Kit
78813-2PCSK9 (His) -LDLR Binding Assay Kit
78813-1PCSK9 (His) -LDLR Binding Assay Kit
78812PCSK9-LDLR Homogeneous Assay Kit
72010PCSK9-LDLR TR-FRET Assay Kit
72011PCSK9 (D374T) -LDLR TR-FRET Assay Kit
82851C-Myc: WDR5 [Biotinylated] Binding Chemiluminescent Assay Kit
78805VHL Binding Assay Kit

Alternative Products

CatalogName
GWB-PS6C8FPCSK9-LDLR Binding Assay Kit
171945PCSK9 LDLR Binding BioAssayTM Kit (Biotin)
172341PCSK9 LDLR Binding BioAssayTM Kit (Pcsk9, Cholesterol, LDL, Kexin) (Biotin) discontinued
EP-103-96testsPCSK9 [Biotinylated] : LDL R Inhibitor Screening ELISA Assay Pair
LMXL2255Mouse XL LDLR Luminex Performance Assay
X2404PRabbit anti Human Proprotein convertase subtilisin/kexin type 9 (PCSK9), LDLR-binding epitope blocking
GTR17701975Rabbit Human Proprotein convertase subtilisin/kexin type 9 (PCSK9), LDLR-binding epitope blocking Antibody
LKU603385Low Density Lipoprotein Receptor (LDLR) Magnetic Luminex Assay Kit
MBS2156781-01Low Density Lipoprotein Receptor (LDLR) Magnetic Fluorescence Assay Kit
BYB64308-01BD140 [for Albumin binding assay]

Popular Products