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PCSK9 (His) -LDLR Binding Assay Kit

CAT: 0421-78813-1Size: 96 ReactionsDry Ice: YesHazardous: No
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CAT#:0421-78813-1Size:96 Reactions
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
PCSK9 (Proprotein convertase subtilisin/kexin type 9) functions as a negative regulator of hepatic low-density lipoprotein receptors (LDLRs) and therefore is a critical regulator of cholesterol metabolism. It is an endopeptidase that binds to the EGFR-like ectodomain of LDLR, leading to LDLR degradation, which in turn, results in increased circulating LDL. Inhibiting the PCSK9-LDLR interaction is an increasingly desirable therapeutic approach for lowering LDL-cholesterol levels to replace or supplement statins. New therapies are critical for addressing atherosclerosis, stroke, heart disease, and other cardiovascular disorders.
Description
The PCSK9 (His) -LDLR Binding Assay Kit is an ELISA-type 96-well format assay designed for screening and profiling purposes. Moreover, two pre-formulated assay buffers are supplied to validate PCSK9-LDLR binding affinity in either neutral or acidic binding conditions. The assay takes advantage of the high sensitivity of detection of His-tag PCSK9 by Anti-His HRP-antibody. First, the LDLR ectodomain is coated on a 96-well plate. Next, PCSK9 is incubated with LDLR on the plate. Finally, the plate is treated with Anti-His HRP-antibody followed by addition of an HRP substrate to produce chemiluminescence, measured using a chemiluminescence reader.Figure 1: Illustration of the ELISA-based PCSK9 (His) -LDLR Binding Assay Kit.Need us to run inhibitor screens or profile your compounds against PCSK9 (His) -LDLR? Check out ourPCSK9 Screening Services.
UniProt
PCSK9: Q8NBP7; LDLR: P01130
Applications
Screen small molecules and antibodies that inhibit the binding of PCSK9 to LDLR ectodomain.
Format
Catalog #NameAmountStorage71204PCSK9 (His) *10 µg-80°C71205LDLR*10 µg-80°CAnti-His HRP-D Antibody-D10 µl-80°C332983x PL-01 Assay Buffer50 ml-20°C797273x PL-02 Assay Buffer50 ml-20°C79728Blocking Buffer 250 ml+4°C79670ELISA ECL Substrate A (translucent bottle) 6 mlRoom TempELISA ECL Substrate B (brown bottle) 6 mlRoom Temp79699White 96-well microplate1Room Temp*The concentration of the protein is lot-specific and will be indicated on the tube.
Shipping Conditions
-80°C
Storage Conditions
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed.
Notes
Troubleshooting GuideVisitbpsbioscience.com/assay-kits-faqfor detailed troubleshooting instructions. For all further questions, please email[email protected]
Contraindications
PCSK9 (His) -LDLR Binding Assay Kit is compatible with up to 1% final DMSO concentration. We recommend preparing the inhibitor in no higher than 10% DMSO solution in buffer and using 5 µl per well.

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