L3MBTL1, GST-tag Recombinant

• Background:

  Polycomb group (PcG) protein that specifically recognizes and binds mono- and dimethyl-lysine residues on target proteins, therey acting as a reader of a network of post-translational modifications. PcG proteins maintain the transcriptionally repressive state of genes: acts as a chromatin compaction factor by recognizing and binding mono- and dimethylated histone H1b/HIST1H1E atLys26 (H1bK26me1 and H1bK26me2) and histone H4 at Lys-20 (H4K20me1 and H4K20me2), leading to condense chromatin and repress transcription. Recognizes and binds p53/TP53 monomethylated at Lys-382, leading to repress p53/TP53-target genes. Also recognizes and binds RB1/RB monomethylated at Lys-860. Participates in the ETV6-mediated repression. Probably plays a role in cell proliferation. Overexpression induces multinucleated cells, suggesting that it is required to accomplish normal mitosis.

• Description:

  Human lethal (3) malignant brain tumor-like protein 1 (L3MBTL1), GenBank Accession No. NM_015478, a.a. 191-530 with N-terminal GST-tag, MW = 65.5 kDa, expressed in anE. colicell expression system.

• UniProt:

  Q9Y468

• Tag:

  N-terminal GST-tag

• Applications:

  Useful for the study of Polycomb group (PcG) proteins, protein binding assays, screening inhibitors, and selectivity profiling.

• Purity:

  ≥ 88%

• Format:

  Aqueous buffer solution

• Shipping Conditions:

  -80°C

• Storage Conditions:

  ≥ 6 months at -80°C

• Calculated Molecular Weight:

  65.5 kDa

• Formulation:

  40 mM Tris-HCl, pH 8.0,110 mM NaCl, 2.2 mM KCl, 1 mM DTT, 20 mM glutathione, and 20% glycerol