Anti-CD325 PE-Cy™7

• Description:

  CD325 (N-cadherin) is a type I transmembrane protein, which forms a complex with catenins, that is linked to the actin cytoskeleton. This complex is important in synapses and for functional plasticity of neurons, and is also essential for embryonic development. Decreased CD325 cleavage caused by mutations in presenilin 1 is associated with Alzheimer´s disease. Besides nervous system, CD325 is expressed on the surface of malignant T cells, and increases their adhesion to epithelia, as well as their ability to invade and metastasize to inflammatory sites.

• Certification:

  RUO

• Immunogen:

  Bacterially expressed extracellular domain of human CD325

• Target Antigen:

  CD325

• Clone:

  8C11

• Applications:

  FC (QC tested)

• Format:

  PE-Cy™7

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Navarro P, Ruco L, Dejana E: Differential localization of VE- and N-cadherins in human endothelial cells: VE-cadherin competes with N-cadherin for junctional localization. J Cell Biol. 1998 Mar 23;140 (6) :1475-84., URL: https://pubmed.ncbi.nlm.nih.gov/9508779/, *Kim JB, Islam S, Kim YJ, Prudoff RS, Sass KM, Wheelock MJ, Johnson KR: N-Cadherin extracellular repeat 4 mediates epithelial to mesenchymal transition and increased motility. J Cell Biol. 2000 Dec 11;151 (6) :1193-206., URL: https://pubmed.ncbi.nlm.nih.gov/11121435/, *Puch S, Armeanu S, Kibler C, Johnson KR, Müller CA, Wheelock MJ, Klein G: N-cadherin is developmentally regulated and functionally involved in early hematopoietic cell differentiation. J Cell Sci. 2001 Apr;114 (Pt 8) :1567-77., URL: https://pubmed.ncbi.nlm.nih.gov/11282032/, *Wahl JK, Kim YJ, Cullen JM, Johnson KR, Wheelock MJ: N-cadherin-catenin complexes form prior to cleavage of the proregion and transport to the plasma membrane. J Biol Chem. 2003 May 9;278 (19) :17269-76., URL: https://pubmed.ncbi.nlm.nih.gov/12604612/, *Jaggi M, Wheelock MJ, Johnson KR: Differential displacement of classical cadherins by VE-cadherin. Cell Commun Adhes. 2002 Mar-Apr;9 (2) :103-15., URL: https://www.ncbi.nlm.nih.gov/pubmed/12487411

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  The mouse monoclonal antibody 8C11 recognizes an extracellular epitope of CD325 (N-cadherin), a transmembrane protein associated with actin cytoskeleton system, which is expressed mainly by neurons, osteoblasts, endothelial cells, and stem cells.

• Applications Notes:

  6

• Species:

  Mouse, Human

• CAS Number:

  9007-83-4

• Isotype:

  Mouse IgG1 kappa