CD23 Antibody

• Description:

  CD23 (FCE2) is a type II integral membrane glycoprotein that is expressed on mature B cells, monocytes, eosinophils, platelets and dendritic cells. CD23 is a low affinity IgE receptor that mediates IgE-dependent cytotoxicity and phagocytosis by macrophages and eosinophils.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P06734

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 48-321 was used as the immunogen for the CD23 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  FCER2/6887

• Applications:

  IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the CD23 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This CD23 antibody is available for research use only.

• Storage Conditions:

  Aliquot the CD23 antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD23 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell Surface

• Image Legend:

  IHC staining of FFPE human tonsil tissue with CD23 antibody (clone FCER2/6887) at 2ug/ml in PBS for 30min RT. Membrane staining observed. Negative control inset: PBS used instead of primary antibody to control for secondary Ab binding. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.