RBP Antibody

• Description:

  Recognizes a protein of 21kDa-25kDa, identified as retinol binding protein (RBP) . Its epitope localizes between aa 74-182 of human RBP. This MAb recognizes reduced and carboxy-methylated RBP (RCM-RBP) as well as the circulatory RBP but not the native RBP, thereby suggesting that its epitope becomes accessible either on unfolding or upon binding of RBP to transthyretin (prealbumin) . RBP is responsible for distributing retinol from the retinoid stores in the liver to the various target tissues. Once secreted into the blood with bound retinol, the vitamin carrier circulates complexed with transthyretin prior to vitamin delivery at the plasma membrane through a receptor-mediated mechanism.

• Specifications:

  The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.

• UniProt:

  P02753, P09455, P50120

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Retinol binding protein purified from human plasma was used as the immunogen for this RBP antibody. Its epitope localizes between amino acids 74-182 of human RBP.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  SPM442

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  Prediluted in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide; *For IHC use only*

• Reconstitution:

  Store the RBP antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This RBP antibody is available for research use only.

• Storage Conditions:

  Store the RBP antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  Prediluted in 1X PBS, 0.1 mg/mL BSA (US sourced), 0.05% sodium azide; *For IHC use only*

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  IHC: Formalin-fixed, paraffin-embedded human hepatocellular carcinoma stained with RBP (clone SPM442) .