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P27Kip1 Antibody

CAT: 0800-V9001-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V9001-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This mAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. p27Kip1 functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P46527
Host
Mouse
Reactivity
Human
Immunogen
Mouse p27Kip1 protein was used as the immunogen for this p27 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
SPM348
Applications
WB, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the p27Kip1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This p27Kip1 antibody is available for research use only.
Storage Conditions
Store the p27Kip1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Nuclear
Image Legend
Immunofluorescent staining of PFA fixed human MCF7 cells with p27Kip1 antibody (clone SPM348, green) and Phalloidin (red) .

UniProtKB · P46527

Cyclin-dependent kinase inhibitor 1B

CDN1B_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P46527
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CDKN1B
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
Q16307, Q5U0H2, Q9BUS6
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Biological process

Cell cycle

Cellular component

CytoplasmEndosomeNucleus

PTM

PhosphoproteinUbl conjugation

Molecular function

Protein kinase inhibitor

Disease

Tumor suppressor

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