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P27K1 Antibody

CAT: 0800-V2436CF488-100TSize: 100 TestsDry Ice: NoHazardous: No
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CAT#:0800-V2436CF488-100TSize:100 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This MAb recognizes a 27kDa protein, identified as the p27Kip1, a cell cycle regulatory mitotic inhibitor. It is highly specific and shows no cross-reaction with other related mitotic inhibitors. In Western blotting of cell lysates from 7 human breast cancer cell lines (ZR75-1, ZR75-30, MCF-7, MDAMB453, T47D, CAL51, 734B), the antibody labels a single band corresponding to p27Kip1. It functions as a negative regulator of G1 progression and has been proposed to function as a possible mediator of TGF- induced G1 arrest. p27Kip1 is a candidate tumor suppressor gene. Reportedly, low p27 expression has been associated with unfavorable prognosis in renal cell carcinoma, colon carcinoma, breast carcinomas, non-small-cell lung carcinoma, hepatocellular carcinoma, multiple myeloma, and lymph node metastases in papillary carcinoma of the thyroid, as well as a more aggressive phenotype in carcinoma of the cervix.
CAS Number
9007-83-4
Specifications
Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood, Immunofluorescence: 1-2 µg/mL
UniProt
P46527
Host
Mouse
Reactivity
Human, Mouse, Rat
Immunogen
Recombinant human protein was used as the immunogen for the p27Kip1 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
KIP1/769
Applications
FACS, IF
Purity
Protein G affinity chromatography
Format
CF488 Conjugate
Buffer
500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the p27K1 antibody at 2-8oC, protected from light.
Limitations
This p27K1 antibody is available for research use only.
Storage Conditions
Store the p27K1 antibody at 2-8°C, protected from light.
Formulation
500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the p27K1 antibody should be determined by the researcher.
Location
Nuclear
Image Legend
Flow cytometry testing of permeabilized human Jurkat cells. Black: cells alone; Grey: isotype control; Green: p27Kip1 antibody (clone KIP1/769) .
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