Biotin Antibody

• Description :

  It recognizes both the free and protein-conjugated (either soluble or cell bound) form of biotin. This MAb is highly specific to biotin and shows no cross-reaction with other structurally related compounds. It has a very high affinity for biotin and is excellent for use in various amplification techniques. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.

• Specifications :

  Flow cytometry: 0.5-1ug/million cells, Immunofluorescence: 0.5-1 µg/mL, Western blot: 0.5-2 µg/mL, Immunohistochemistry (FFPE) : 0.5-1 µg/mL

• UniProt :

  Not Applicable

• Host :

  Mouse

• Reactivity :

  All Species Expected

• Immunogen :

  Biotinylated sheep immunoglobulin was used as the immuongen for the Biotin antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  BTN399

• Applications :

  FACS, IF, WB, IHC-P

• Purity :

  Protein A/G affinity

• Format :

  Purified

• Buffer :

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution :

  Aliquot the Biotin antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This Biotin antibody is available for research use only.

• Storage Conditions :

  Aliquot the Biotin antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the Biotin antibody should be determined by the researcher.

• CAS Number :

  9007-83-4

• Image Legend :

  IHC staining of endogenous biotin in FFPE human kidney tissue with Biotin antibody (clone BTN399) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.