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Recombinant EpCAM Antibody

CAT: 0800-V3464-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V3464-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Binding epitope of this antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59 of Ep-CAM. EGP40 is a 40-43kDa transmembrane epithelial glycoprotein, also identified as epithelial specific antigen (ESA), or epithelial cellular adhesion molecule (Ep-CAM) . It is expressed on baso-lateral cell surface in most simple epithelia and a vast majority of carcinomas with the exception of adult squamous epithelium, hepatocytes and gastric epithelial cells. This antibody has been used to distinguish adenocarcinoma from pleural mesothelioma and hepatocellular carcinoma. This antibody is also useful in distinguishing serous carcinomas of the ovary from mesothelioma.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 0.5-1 µg/mL for 30 min at RT, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Flow cytometry: 1-2ug/million cells
UniProt
P16422
Host
Mouse
Reactivity
Human
Immunogen
Neuraminidase treated GLS-1 human small cell lung carcinoma cells were used as the immunogen for the recombinant EpCAM antibody. Binding epitope of the rMOC-31 antibody is located in the first EGF-like repeat domain (EGF1) between amino acids 27-59.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RMOC-31
Type
Recombinant
Applications
IHC-P, IF, WB, FACS
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant EpCAM antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant EpCAM antibody is available for research use only.
Storage Conditions
Store the recombinant EpCAM antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant EpCAM antibody should be determined by the researcher.1. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
Location
Cell surface, cytoplasmic
Image Legend
Immunofluorescence testing of human MCF7 cells with recombinant EpCAM antibody (green, clone rMOC-31) and RedDot nuclear stain (red) .

UniProtKB · P16422

Epithelial cell adhesion molecule

EPCAM_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P16422
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EPCAM
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Ep-CAM
EC number
—
Processing
Precursor
Secondary accessions
P18180, Q6FG26, Q6FG49, Q96C47, Q9UCD0
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Cellular component

Cell junctionCell membraneMembraneTight junction

Disease

Disease variantHereditary nonpolyposis colorectal cancer

PTM

Disulfide bondGlycoprotein

Domain

RepeatSignalTransmembraneTransmembrane helix

Molecular function

Tumor antigen

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