Recombinant SDHB Antibody

• Description :

  Succinate dehydrogenase B (SDHB) is an iron-sulfur subunit of mitochondrial complex II, a key component of the citric acid cycle and the electron transport chain, a respiratory complex that catalyzes the oxidation of succinate in the mitochondrial membrane. Many cancers are generally positive for SDHB, including renal cell carcinomas and gastrointestinal stromal tumors. A subset of RCC and GIST tumors that are associated with SDH mutations, Carney-Stratakis Syndrome or Carney Triad exhibit a loss of SDHB expression.

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt :

  P21912

• Host :

  Rabbit

• Reactivity :

  Human

• Immunogen :

  A synthetic peptide specific to human SDHB was used as the immunogen for the recombinant SDHB antibody.

• Clonality :

  Recombinant Monoclonal

• Isotype :

  IgG

• Clone :

  SDHB/6697R

• Type :

  Recombinant

• Applications :

  IHC-P

• Purity :

  Protein A/G affinity

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Aliquot the recombinant SDHB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations :

  This recombinant SDHB antibody is available for research use only.

• Storage Conditions :

  Aliquot the recombinant SDHB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  Optimal dilution of the recombinant SDHB antibody should be determined by the researcher.

• CAS Number :

  9000-83-3

• Location :

  Cytoplasm

• Image Legend :

  IHC staining of FFPE human heart tissue with recombinant SDHB antibody (clone SDHB/6697R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.