SDHB Antibody

• Description:

  Succinate dehydrogenase (SDH) is Complex II in the mitochondria, vital for mitochondrial electron transport, as well as Krebs cycle function.Four subunits comprise the SDH protein complex: a flavochrome subunit (SDHA), an iron-sulfur protein (SDHB) and two membrane-bound subunits (SDHC and SDHD) anchored to the inner mitochondrial membrane. The SDH complex functions as a tumor suppressor. Loss of any subunit proteins lead to destabilization of the complex and tumor formation.Antibody to SDHB is helpful in the identification of phaeochromocytomas, paragangliomas and GIST.

• Specifications:

  Western blot: 0.5-1 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P21912

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A portion of amino acids 165-273 was used as the immunogen for the SDHB antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  SDHB/2126

• Applications:

  WB, IHC-P

• Purity:

  Protein A/G affinity

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Aliquot the SDHB antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

• Limitations:

  This SDHB antibody is available for research use only.

• Storage Conditions:

  Aliquot the SDHB antibody and store frozen at -20°C or colder. Avoid repeated freeze-thaw cycles.

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the SDHB antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic

• Image Legend:

  Western blot testing of human 1) 293, 2) Jurkat and 3) HepG2 cell lysate using SDHB antibody (clone SDHB/2126) .