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CD27 Antibody

CAT: 0800-V8725-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V8725-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NF B and SAPK/JNK and induces apoptosis.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT, Western blot: 2-4 µg/mL
UniProt
P26842
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 28-170 from the human protein was used as the immunogen for the CD27 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
LPFS2/4177
Applications
IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the CD27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD27 antibody is available for research use only.
Storage Conditions
Store the CD27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD27 antibody should be determined by the researcher.
Location
Cell surface
Image Legend
IHC staining of FFPE human tonsil with CD27 antibody (clone LPFS2/4177) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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