DAZL Antibody

• Description:

  Specific for human DAZL, a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is localized to the cytoplasm. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Clone DAZL/4253 recognizes DAZL at its C terminus, and is used in detection of DAZL expression by various methods.

• Specifications:

  Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt:

  Q92904

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Amino acids CRVHHFRRSRAMLKSV from the human protein (C-terminal region) was used as the immunogen for the DAZL antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1

• Clone:

  DAZL/4253

• Applications:

  IF, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the DAZL antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This DAZL antibody is available for research use only.

• Storage Conditions:

  Store the DAZL antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the DAZL antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, nuclear

• Image Legend:

  IHC staining of FFPE human testis with DAZL antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.