CD15 Antibody

• Description:

  CD15 plays a role in mediating phagocytosis, bactericidal activity, and chemotaxis. It is present on 95% of granulocytes including neutrophils and eosinophils and to a lesser degree on monocytes. In addition, CD15 is expressed in Reed-Sternberg cells and some epithelial cells. CD15 antibody is very useful in the identification of Hodgkin s disease. CD15 is occasionally expressed in large cell lymphomas of both B and T phenotypes which otherwise have a quite distinct histological appearance.

• Specifications:

  Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

• UniProt:

  P22083

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Purified neutrophils from normal human peripheral blood were used as the immunogen for the CD15 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgM k

• Clone:

  MY-1

• Applications:

  IF, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the CD15 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD15 antibody is available for research use only.

• Storage Conditions:

  Store the CD15 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the CD15 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface and granular paranuclear

• Image Legend:

  Immunofluorescent staining of human U937 cells with CD15 antibody (clone MY-1, green) and Reddot nuclear stain (red) .