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Fibronectin Antibody

CAT: 0800-V8431SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V8431SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Fibronectins are disulfide-linked, dimeric glycoproteins of ~440kDa. They possess at least four binding sites for collagen, glycosaminoglycans, transglutaminase, and a cell surface receptor. Epitope of this MAb is located in the 2nd-3rd type-III repeats of fibronectin. Fibronectins are extracellular matrix glycoproteins that are essential for embryonic development. Fibronectins are also involved in cell adhesion, tissue organization, and wound healing. Fibronectins are present in basement membranes, interstitial connective tissue matrix, and blood. Cellular fibronectin is widely distributed in the stroma of many malignant tumors. This MAb reacts with human cellular fibronectin, but not plasma fibronectin.
CAS Number
9007-83-4
Specifications
ELISA: order Ab without BSA for coating, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P02751
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 467-595 from the human protein was used as the immunogen for the Fibronectin antibody.
Clonality
Monoclonal
Isotype
IgG κ
Clone
FN1/3029
Applications
ELISA, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the Fibronectin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Fibronectin antibody is available for research use only.
Storage Conditions
Store the Fibronectin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the Fibronectin antibody should be determined by the researcher.
Location
Connective tissue matrix
Image Legend
IHC staining of FFPE human lung with Fibronectin antibody (clone FN1/3029) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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