Products for Research Use Only

Nidogen Antibody

CAT: 0800-V8402-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V8402-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Entactin/Nidogen is a ubiquitous multi-domain basement membrane protein. Basement membranes are the earliest extracellular matrices produced during embryogenesis. They are synthesized and incorporated into the supramolecular architecture of several components, including laminins, Collagen IV, Nidogen and proteoglycans. Nidogen/Entactin, a sulfated glycoprotein, acts as a link between the extracellular matrix molecules Laminin 1 and Collagen Type IV, and thereby participates in the assembly of basement membranes. Nidogen is synthesized and secreted in primary and established mesenchymal peritubular cells and myoepithelial cells, and it affects adhesion of peritubular cells in an autocrine manner. Nidogen is expressed during embryonic and fetal development exclusively in fully developed basement membranes of the ectoderm and is not expressed in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation. This MAb does not cross-react with other basement membrane components or fibronectin.
CAS Number
9007-83-4
Specifications
ELISA: 2-4 µg/mL for coating; order Ab without BSA
UniProt
P10493
Host
Rat
Reactivity
Mouse
Immunogen
A murine EHS laminin preparation was used as the immunogen for the Nidogen antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
ELM1
Applications
ELISA
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Nidogen antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Nidogen antibody is available for research use only.
Storage Conditions
Store the Nidogen antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Nidogen antibody should be determined by the researcher.
Location
Basement membrane
Image Legend
SDS-PAGE analysis of purified, BSA-free Nidogen antibody (clone ELM1) as confirmation of integrity and purity.