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Recombinant Villin Antibody

CAT: 0800-V8401SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V8401SAF-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of 95kDa, which is identified as villin. It is a major constituent in the microvilli, which compose the brush border of epithelial cells forming absorptive surfaces of the intestinal and renal proximal tubular epithelia. Anti-Villin labels the brush border area in the gastrointestinal mucosal epithelium and urogenital tract. Among neoplasms, villin is predominantly expressed in tumors of colorectal origin. Antibody to villin is useful in identifying malignant cells from primary and metastatic colorectal carcinomas. This antibody also labels Merkel cells of the skin.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P09327
Host
Rabbit
Reactivity
Human
Immunogen
A portion of amino acids 600-700 from the human protein was used as the immunogen for the recombinant Villin antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG
Clone
VIL1/4107R
Type
Recombinant
Applications
IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the recombinant Villin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant Villin antibody is available for research use only.
Storage Conditions
Store the recombinant Villin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the recombinant Villin antibody should be determined by the researcher.
Location
Cytoplasmic & Cell surface
Image Legend
IHC staining of FFPE human colon with recombinant Villin antibody (clone VIL1/4107R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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