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Recombinant CD74 Antibody

CAT: 0800-V8374-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V8374-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
It recognizes a protein of ~35kDa, identified as CD74 (Workshop IV) . CD74 is a type II transmembrane protein which binds to the peptide binding groove of newly synthesized MHC class II alpha/beta heterodimers and prevents their premature association with endogenous polypeptides. CD74 is expressed primarily by antigen presenting cells, such as B-lymphocytes (from before the pre-B cell stage to before the plasma cell stage), macrophages, and monocytes, and many epithelial cells. Anti-CD74 stains predominantly germinal center lymphocytes and B-cell lymphomas, but rarely T-cell lymphomas. Anti-CD74 has been shown to be useful in differentiating atypical fibroxanthoma (-) from malignant fibrous histiocytoma (+) .
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT
UniProt
P044233
Host
Mouse
Reactivity
Human
Immunogen
Recombinant full-length human CD74 protein was used as the immunogen for the recombinant CD74 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RCLIP/813
Type
Recombinant
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant CD74 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant CD74 antibody is available for research use only.
Storage Conditions
Store the recombinant CD74 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant CD74 antibody should be determined by the researcher.
Location
Cell surface and paranuclear globular
Image Legend
IHC staining of FFPE human tonsil with recombinant CD74 antibody (clone rCLIP/813) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.
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