Recombinant COX-2 Antibody

Prostaglandins are a diverse group of autocrine and paracrine hormones that mediate many cellular and physiologic processes. Prostaglandin H2 (PGH2) is an intermediate molecule in formation of the prostaglandins. Cyclooxygenase-1 (Cox-1) and cyclooxygenase-2 (Cox-2) are prostaglandin synthases that catalyze the formation of PGH2 from arachidonic acid (AA) . Cox-1 and Cox-2 are isozymes of prostaglandin-endoperoxidase synthase (PTGS) . Cox-1 is constitutively expressed in most tissues and is thought to serve in general housekeeping functions. Cox-2 is efficiently induced in migratory cells responding to pro-inflammatory stimuli and is considered to be an important mediator of inflammation. Both enzymes are targets for the nonsteroidal therapeutic anti-inflammatory drugs, NSAIDs. COX2 expression is significantly increased in 85-90% of human colorectal adenocarcinomas whereas levels of COX-1 are not changed.

9000-83-3

Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT

P35354

Human

A portion of amino acids 504-604 from the human protein was used as the immunogen for the recombinant COX-2 antibody.

COX2/3320R

Recombinant

IHC-P

Protein A affinity chromatography

Purified

1 mg/ml in 1X PBS; BSA free, sodium azide free

Store the recombinant COX-2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

This recombinant COX-2 antibody is available for research use only.

Store the recombinant COX-2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

1 mg/mL in 1X PBS; BSA free, sodium azide free

Optimal dilution of the recombinant COX-2 antibody should be determined by the researcher.

Cytoplasm, Cell surface