PD1 Antibody / PDCD1

• Description:

  PD-1 is expressed on a subset of CD4-CD8-thymocytes, and on activated T and B cells. PD-1 is thought to be involved in lymphocyte clonal selection and peripheral tolerance. The PD-1 ligands, PD-L1 (also known as B7-H1) and PD-L2 (B7-DC), are members of the B7 immunoglobulin superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B-lymphocytes and myeloid cells. Additionally, Pdcd1 mRNA is expressed in developing B-lymphocytes during the pro-B-cell stage. The RMP1-14 antibody has been reported to block the binding of PD-1 to its ligands (B7-H1 and B7-DC) and to inhibit T cell proliferation and cytokine production co-stimulated by macrophages (but not by dendritic cells and B cells) .

• Specifications:

  ELISA (order BSA/azide-free format for coating), Neutralization Studies (order BSA/azide-free format), Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunofluorescence: 0.5-1 µg/mL

• UniProt:

  Q02242

• Host:

  Rat

• Reactivity:

  Mouse

• Immunogen:

  PDCD1 transfected BHK cells were used as the immunogen for the PD1 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  RMP1-14

• Applications:

  ELISA, Neutralization, FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the PD1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This PD1 antibody is available for research use only.

• Storage Conditions:

  Store the PD1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the PD1 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  SDS-PAGE analysis of purified, BSA-free PD1 antibody (clone RMP1-14) as confirmation of integrity and purity.