HPV16 E1/E4 Antibody

• Description :

  The human papilloma virus (HPV) family of DNA tumor viruses includes HPV16, a 'high-risk' sexually-transmitted HPV that can lead to cervical, anal, vulvar, head, neck, and penile cancer. The HPV16E1/E4 protein is expressed abundantly in cells supporting viral DNA amplification, but is lost during malignant progression. HPV16E1/E4 causes G2 cell cycle arrest by associating with and preventing the nuclear entry of Cdk1/cyclin B1 complexes. HPV16E1/E4 also interacts with cyclin A and Cdk2 during the G2 phase of the cell cycle, and this association may increase the efficiency with which HPV16E1/E4 is able to prevent mitotic entry. HPV16E1/E4 also associates with keratin intermediate filaments and causes the network to collapse.

• CAS Number :

  9007-83-4

• Specifications :

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• Host :

  Mouse

• Reactivity :

  HPV-16

• Immunogen :

  A recombinant human HPV16 E1/E4 fragment (amino acids 36-41) was used as the immunogen for this HPV16 E1/E4 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  HPV16 E1/E4

• Applications :

  IHC-P

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution :

  Store the HPV16 E1/E4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This HPV16 E1/E4 antibody is available for research use only.

• Storage Conditions :

  Store the HPV16 E1/E4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes :

  Optimal dilution of the HPV16 E1/E4 antibody should be determined by the researcher.

• Location :

  Nuclear

• Image Legend :

  IHC staining of FFPE human cervix with HPV16 E1/E4 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.