Recombinant Interferon gamma Antibody

• Description:

  Recognizes a protein of 20-25kDa, identified as human interferon. This MAb is specific to human IFN-g and recognizes both recombinant and native human IFN-gamma. T lymphocytes and NK cells mainly produce IFN-g. It is a pleiotropic cytokine involved in the regulation of nearly all phases of immune and inflammatory responses, including the activation, growth and differentiation of T cell, B cells, macrophages, NK cells and other cell types such as endothelial cells and fibroblasts. It has weak anti-viral and anti-proliferative activity, and potentiates the antiviral and anti-tumor effects of IFN-a/b (type I interferon) .

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P01579

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Purified recombinant human IFNG was used as the immunogen for the recombinant Interferon gamma antibody.

• Clonality:

  Recombinant Monoclonal

• Isotype:

  IgG κ

• Clone:

  IFNG/3996R

• Type:

  Recombinant

• Applications:

  IHC-P

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the recombinant Interferon gamma antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This recombinant Interferon gamma antibody is available for research use only.

• Storage Conditions:

  Store the recombinant Interferon gamma antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the recombinant Interferon gamma antibody should be determined by the researcher.

• CAS Number:

  9000-83-3

• Location:

  Cytoplasmic, membranous, extracellular (secreted)

• Image Legend:

  IHC staining of FFPE human kidney with recombinant Interferon gamma antibody (clone IFNG/3996R) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.