Products for Research Use Only

Proliferation Marker Antibody

CAT: 0800-V8296-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V8296-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
JC1 detects a nuclear antigen, distinct from Ki67, present in proliferating cells. It is useful as a marker of proliferation in cases such as squamous cell carcinoma where the Ki67 index cannot be determined.
CAS Number
9007-83-4
Specifications
Western blot: 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
Host
Mouse
Reactivity
Human
Immunogen
Pokeweed-stimulated lymphocytes were used as the immunogen for this Proliferation Marker antibody.
Clonality
Monoclonal
Isotype
IgG2a κ
Clone
JC1
Applications
WB, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the Proliferation Marker antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This Proliferation Marker antibody is available for research use only.
Storage Conditions
Store the Proliferation Marker antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the Proliferation Marker antibody should be determined by the researcher.
Location
Nuclear
Image Legend
IHC staining of FFPE human lymph node with Proliferation Marker antibody (clone JC1) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.