CD3e Antibody

• Description:

  CD3 chains. CD3 plays a critical role in TCR signal transduction, T cell activation, and antigen recognition by binding the peptide/MHC antigen complex. The 145-2C11 antibody is useful for in vitro blocking and activation assays, as well as apoptosis induction and in vivo T cell depletion.

• Specifications:

  Functional Studies (order BSA/azide-free format), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL

• UniProt:

  P07766

• Reactivity:

  Mouse

• Immunogen:

  The H2Kb-specific mouse cytotoxic T lymphocyte clone BM10 37 was used as the immunogen for the CD3e antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG κ

• Clone:

  145-2C11

• Applications:

  Functional Studies, FACS, IF

• Purity:

  Protein A affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD3e antibody is available for research use only.

• Storage Conditions:

  Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD3e antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  SDS-PAGE analysis of purified, BSA-free CD3e antibody (clone 145-2C11) as confirmation of integrity and purity.