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CD68 Antibody

CAT: 0800-V8261-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V8261-100UGSize:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody recognizes a glycoprotein of 110kDa, which is identified as CD68. It is important for identifying macrophages in tissue sections. It stains macrophages in a wide variety of human tissues, including Kupffer cells and macrophages in the red pulp of the spleen, in lamina propria of the gut, in lung alveoli, and in bone marrow. It reacts with myeloid precursors and peripheral blood granulocytes. It also reacts with plasmacytoid T cells, which are supposed to be of monocyte/macrophage origin. It shows strong granular cytoplasmic staining of chronic and acute myeloid leukemia and also reacts with rare cases of true histiocytic neoplasia. Lymphomas are negative or show few granules.
CAS Number
9007-83-4
Specifications
Immunohistochemistry (FFPE) : 0.25-0.5 µg/mL
UniProt
P34810
Host
Mouse
Reactivity
Human
Immunogen
A recombinant human partial protein (amino acids 150-221) was used as the immunogen for this CD68 antibody.
Clonality
Monoclonal
Isotype
IgG2b κ
Clone
C68/2511
Applications
IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the CD68 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD68 antibody is available for research use only.
Storage Conditions
Store the CD68 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD68 antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
IHC staining of FFPE human tonsil with CD68 antibody (clone C68/2511) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

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