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CD27 Antibody

CAT: 0800-V8229SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V8229SAF-100UGSize:100 µg
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Recognizes a protein of a disulfide-linked 120kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFB and SAPK/JNK and induces apoptosis.
CAS Number
9007-83-4
Specifications
ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunofluorescence: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL
UniProt
P26842
Host
Mouse
Reactivity
Human
Immunogen
A recombinant full-length human CD27 protein was used as the immunogen for this CD27 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
LPFS2/1611
Applications
ELISA, FACS, IF, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the CD27 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD27 antibody is available for research use only.
Storage Conditions
Store the CD27 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Applications Notes
Optimal dilution of the CD27 antibody should be determined by the researcher.
Image Legend
Immunofluorescent staining of human Ramos cells with CD27 antibody (clone LPFS2/1611, green) and Reddot nuclear stain (red) .

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