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CD19 Antibody

CAT: 800-V8223-20UGSize: 20 µgDry Ice: NoHazardous: No
20 µgSelected
PRICE
AVAILABILITY
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is present in both benign and malignant B-cells and is considered to be the most reliable surface marker of this lineage over a wide range of maturational stages. In normal lymphoid tissue, CD19 is observed in germinal centers, in mantle zone cells, and in scattered cells of the inter-follicular areas. Anti-CD19 exhibits an overall immunoreactivity pattern similar to those of the antibodies against CD20 and CD22. However, in contrast to CD20, expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. Anti-CD19 positivity is seen in the vast majority of B-cell neoplasms commonly at a lower intensity than normal B-cell counterparts. Plasma cell neoplasms are nearly always negative, as are T-cell neoplasms.
CAS Number
9007-83-4
Specifications
Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL
UniProt
P15391
Host
Mouse
Reactivity
Human
Immunogen
Pokeweek-stimulated Daudi and Raji cells were used as the immunogen for the CD19 antibody
Clonality
Monoclonal
Isotype
IgM k
Clone
PDR134
Applications
FACS, IF, WB
Purity
PEG precipitation
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the CD19 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD19 antibody is available for research use only.
Storage Conditions
Store the CD19 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the CD19 antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
Flow cytometry testing of lymphocyte-gated human PBM cells with CD19 antibody (clone PDR134) ; Gray=unstained cells, Red= anti-CD19 antibody stained cells.

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