CD3e Antibody

• Description:

  Recognizes the epsilon-chain of CD3, which consists of five different polypeptide chains (designated as gamma, delta, epsilon, zeta, and eta) with MW ranging from 16-28kDa. It is found on all mature T-lymphocytes, NK, T-cells, and some thymocytes. CD3, also known as T3, is a member of the immunoglobulin superfamily that plays a role in antigen recognition, signal transduction, and T cell activation. It is expressed, typically at high levels, on peripheral T cells and majority of T cell neoplasms. Thymocytes express CD3 at different level on the cell surface in the course of differentiation and, in cortical thymus, CD3 is predominantly Intracytoplasmic. This MAb has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection.

• Specifications:

  Flow cytometry: 1-2ug/10^6 cells, Immunohistochemistry (acetone fixed frozen sections), Immunofluorescence: 1-2 µg/mL, Functional Studies (order BSA/azide-free format)

• UniProt:

  P07766

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Human PBMC were used as the immunogen for the CD3e antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2a κ

• Clone:

  OKT3

• Applications:

  FACS, ICC, IF, Functional Studies

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the CD3e antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD3e antibody is available for research use only.

• Storage Conditions:

  Store the CD3e antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD3e antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic

• Image Legend:

  Flow cytometry staining of lymphocyte-gated human PBM cells with CF640R-labeled CD3e antibody (clone OKT3) . Gray=unstained, Red=CF640R-CD3e antibody.