Alpha Actinin 4 Antibody / ACTN4

• Description:

  The spectrin gene family encodes a diverse group of cytoskeletal proteins that include spectrins, dystrophins and -actinins. There are four tissue-specific -actinins, namely -actinin-1, -actinin-2, -actinin-3 and -actinin-4, which are localized to muscle and non-muscle cells, including skeletal, cardiac and smooth muscle cells, as well as within the cytoskeleton. Each -actinin protein contains one Actin-binding domain, two calponin-homology domains, two EF-hand domains and four spectrin repeats, through which they function as bundling proteins that can cross-link F-Actin, thus anchoring Actin to a variety of intracellular structures. Defects in the gene encoding -actinin-4 are the cause of focal segmental glomerulosclerosis 1 (FSGS1), a common renal lesion characterized by decreasing kidney function and, ultimately, renal failure.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  O43707

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant full-length human ACTN4 protein was used as the immunogen for this Alpha Actinin 4 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG

• Clone:

  93

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the Alpha Actinin 4 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Alpha Actinin 4 antibody is available for research use only.

• Storage Conditions:

  Store the Alpha Actinin 4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the Alpha Actinin 4 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear, cytoplasmic

• Image Legend:

  SDS-PAGE analysis of purified, BSA-free Alpha Actinin 4 antibody (clone 93) as confirmation of integrity and purity.