Products for Research Use Only

Alpha-Actinin Antibody

CAT: 0800-R30092Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-R30092Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Alpha-actinin was initially isolated from rabbit skeletal muscle as a factor that induces the gelation of F-actin and promotes the superprecipitation of actomyosin. Alpha actinins are actin-binding proteins that carry out different purposes in different different cell types. In myofibrillar cells, alpha-actinin constitutes a major component of Z-discs in striated muscle and of the functionally analogous dense bodies and dense plaques in smooth muscle. Alpha-actinin (alpha A) shares structural homology with spectrin and dystrophin.
CAS Number
9007-83-4
Specifications
Western blot: 0.5-1 µg/mL, IHC (FFPE) : 1.5-3 µg/mL, IHC (Frozen) : 1.5-3 µg/mL to detect in formalin or acetone fixed tissues
Host
Mouse
Reactivity
Human, Mouse, Rat, Rabbit
Immunogen
Rabbit skeletal alpha-actinin was used as the immunogen for this alpha-Actinin antibody.
Clonality
Monoclonal
Isotype
IgG1
Clone
EA-53
Applications
WB, IHC-P, IHC-F
Purity
Ascites
Format
Ascites
Buffer
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Reconstitution
After reconstitution, the alpha-Actinin antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20oC. Avoid repeated freezing and thawing.
Limitations
This alpha-Actinin antibody is available for research use only.
Storage Conditions
After reconstitution, the alpha-Actinin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Formulation
0.5 mg/mL with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1X PBS
Applications Notes
The stated application concentrations are suggested starting amounts. Titration of the alpha-Actinin antibody may be required due to differences in protocols and secondary/substrate sensitivity.
Image Legend
Western blot testing of 1) rat heart, 2) rat skeletal muscle, 3) mouse heart, 4) mouse skeletal muscle and 5) human placenta lysate with alpha-Actinin antibody at 0.5ug/ml. Predicted molecular weight ~105 kDa.

ALPHA-ACTININ

Normalized nameALPHA-ACTININ
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