Transferrin Antibody

• Description:

  Iron (Fe) is a tightly metabolically controlled mineral and growth factor present in all living cells. Iron not bound in erythrocyte hemoglobin is transported by transferrin (Tf), the iron transport protein of vertebrate serum. The transferrin protein contains two homologous domains, each of which contain an Fe-binding site. The majority of transferrin is synthesized in the liver and secreted into the blood, but it is also produced in lower amounts in testis and brain as well as in oligodendrocytes, where transferrin is an early marker of oligodendrocyte differentiation. From the blood, transferrin is internalized by erythroblasts and reticulocytes upon binding the transferrin receptor (TfR), also designated CD71, through a system of coated pits and vesicles. After Fe release, transferrin is returned to the extracellular medium, where it can be reused. Defects in the transferrin gene results in atransferrinemia, a rare autosomal recessive disorder characterized by microcytic anemia and iron loading.

• Specifications:

  Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P02787

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant human partial protein (amino acids 311-445) was used as the immunogen for this Transferrin antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  TF/3001

• Applications:

  IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the Transferrin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Transferrin antibody is available for research use only.

• Storage Conditions:

  Store the Transferrin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the Transferrin antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Secreted

• Image Legend:

  IHC staining of FFPE human prostate carcinoma with Transferrin antibody (clone TF/3001) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.