L-Selectin Antibody / CD62L

• Description:

  Selectins, also designated CD62 antigens, comprise a family of carbohydrate-binding proteins involved in mediating cellular interactions with leukocytes. L-Selectin (also designated LECAM-1 or CD62L) is expressed on the majority of B and naive T cells and on most monocytes, neutrophils and eosinophils. L-Selectin interacts with specific carbohydrates expressed by activated endothelial cells. P-Selectin (also designated GMP-140 or CD62P), expressed on activated platelets and endothelial cells, and E-Selectin (also designated ELMA-1 or CD62E), expressed on endothelial cells, exhibit overlapping ligand specificities. Both recognize sialyl-Le (x) as a ligand and bind to specific carbohydrates on neutrophils and monocytes.

• Specifications:

  ELISA (order BSA-free format for coating), Flow cytometry: 1-2ug/10^6 cells in 0.1ml, Western blot: 1-2 µg/mL

• UniProt:

  P14151

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Supernatant from phorbol myristic acid activated human peripheral blood leukocytes was used as the immunogen for the L-Selectin antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  LAM1-116

• Applications:

  ELISA, FACS, WB

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the L-Selectin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This L-Selectin antibody is available for research use only.

• Storage Conditions:

  Store the L-Selectin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the L-Selectin antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Image Legend:

  SDS-PAGE analysis of purified, BSA-free L-Selectin antibody (clone LAM1-116) as confirmation of integrity and purity.