CD45RB Antibody

• Description:

  CD45R, also designated CD45 and PTPRC, has been identified as a transmembrane glycoprotein, broadly expressed among hematopoietic cells. Multiple isoforms of CD45R are distributed throughout the immune system according to cell type. These isoforms arise because of alternative splicing of exons 4, 5, and 6. The corresponding protein domains are characterized by the binding of monoclonal antibodies specific for CD45RA (exon 4), CD45RB (exon 5), CD45RC (exon 6) and CD45RO (exons 4 to 6 spliced out) . The variation in these isoforms is localized to the extracellular domain of CD45R, while the intracellular domain is conserved. CD45R functions as a phosphor-tyrosine phosphatase. Antibody to CD45 is useful in differential diagnosis of lymphoid tumors from non-hematopoietic undifferentiated neoplasms.

• Specifications:

  Flow cytometry: 5ul per test per one 10^6 cells in 0.1ml or 5ul per 100ul of whole blood, Immunofluorescence: 1:50-1:100

• UniProt:

  P08575

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  The non-T, non-B CALLA positive ALL cell line REH (Leucocyte Workshop IV and V) was used as the immunogen for this antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  BRA-11

• Applications:

  FACS, IF

• Purity:

  Protein G affinity chromatography

• Format:

  CF488 Conjugate

• Buffer:

  500 ul at 0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the CD45RB antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This CD45RB antibody is available for research use only.

• Storage Conditions:

  Store the CD45RB antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  500 ul at 0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD45RB antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface, cytoplasmic