MSH2 Antibody

• Description:

  Mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) . Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes MutS and MutL were found at high frequency in HNPCC and were shown to be associated with microsatellite instability. The demonstration that 10 to 45% of pancreatic, gastric, breast, ovarian and small cell lung cancers also display microsatellite instability has been interpreted to suggest that DNA mismatch repair is not restricted to HNPCC tumors but is a common feature in tumor initiation or progression.

• Specifications:

  Flow cytometry: 1-2ug/million cells in 0.1ml, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL, Immunofluorescence: 1-2 µg/mL

• UniProt:

  P43246

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant human partial protein (amino acids 327-427) was used as the immunogen for this MSH2 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  MSH2/2622

• Applications:

  FACS, IF, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the MSH2 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This MSH2 antibody is available for research use only.

• Storage Conditions:

  Store the MSH2 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the MSH2 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Nuclear

• Image Legend:

  IHC staining of FFPE human colon carcinoma with MSH2 antibody. HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.