CD32 Antibody

• Description :

  This CD32 antibody binds most strongly to the IIa isoforms of CD32 (FcRII) , a type 1 transmembrane glycoprotein that mediates several functions including phagocytosis, cytotoxicity, and immunomodulation as well as platelet aggregation. Three genes (A, B, and C) encode CD32 and at least 6 isoforms are generated via alternative mRNA splicing, i.e., IIa1, IIa2, IIb1, IIb2, IIb3 and IIc. Monocytes/macrophages, placental trophoblasts and endothelial cells express all isoforms. In addition, the IIb isoform is expressed by B cells, and the IIa isoform by platelets, granulocytes and, weakly, by B cells. NK cells and neutrophils express Isoform IIc. CD32 binds weakly to the Fc region of monomeric IgG but more strongly to IgG aggregates and immune complexes.

• Specifications :

  Flow cytometry: 1-2ug/10^6 cells in 0.1ml

• UniProt :

  P12318

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  Recombinant human protein was used as the immunogen for the CD32 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG2b κ

• Clone :

  FCGR2A/479

• Applications :

  FACS

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution :

  Store the CD32 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This CD32 antibody is available for research use only.

• Storage Conditions :

  Store the CD32 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes :

  Optimal dilution of the CD32 antibody should be determined by the researcher.

• CAS Number :

  9007-83-4