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Recombinant AKT1 Antibody

CAT: 0800-V7745-100UGSize: 100 µgDry Ice: NoHazardous: No
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CAT#:0800-V7745-100UGSize:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
The serine/threonine kinase Akt family contains several members, including Akt1 (also designated PKB or RacPK), Akt2 (also designated PKB tyrosine residues 740 and 751, which bind the subunit of the phosphatidylinositol 3-kinase (PI 3-kinase) complex. Activation of Akt1 by insulin or insulin-growth factor-1 (IGF-1) results in phosphorylation of both Thr 308 and Ser 473. Akt proteins become phosphorylated and activated in insulin/IGF-1-stimulated cells by an upstream kinase (s), and the activation of Akt1 and Akt2 is inhibited by the PI kinase inhibitor wortmannin.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL, Western blot: 1-2 µg/mL
UniProt
P31749
Host
Mouse
Reactivity
Human
Immunogen
Amino acids 85-189 from the human protein were used as the immunogen for the recombinant AKT1 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG1 k
Clone
RAKT1/2491
Type
Recombinant
Applications
IHC-P, WB
Purity
Protein G affinity chromatography
Format
Purified
Buffer
0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the recombinant AKT1 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant AKT1 antibody is available for research use only.
Storage Conditions
Store the recombinant AKT1 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Applications Notes
Optimal dilution of the recombinant AKT1 antibody should be determined by the researcher.
Location
Nucleus, plasma membrane, cytoplasm
Image Legend
IHC staining of FFPE human prostate carcinoma with recombinant AKT1 antibody (clone rAKT1/2491) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min followed by cooling at RT for 20 min.

UniProtKB · P31749

RAC-alpha serine/threonine-protein kinase

AKT1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P31749
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
AKT1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
2.7.11.1
Processing
—
Secondary accessions
B2RAM5, B7Z5R1, Q9BWB6
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationDisulfide bondGlycoproteinIsopeptide bondPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

ApoptosisCarbohydrate metabolismGlucose metabolismGlycogen biosynthesisGlycogen metabolismNeurogenesisSugar transportTranslation regulationTransport

Ligand

ATP-bindingNucleotide-binding

Cellular component

Cell membraneCytoplasmMembraneMitochondrionNucleus

Molecular function

Developmental proteinKinaseSerine/threonine-protein kinaseTransferase

Disease

Disease variantProto-oncogene

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