CD73 Antibody

CAT: 0800-V7588SAF-100UGSize: 100 µgDry Ice: NoHazardous: No
CAT#:0800-V7588SAF-100UGSize:100 µg
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI) -anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.
CAS Number
9007-83-4
Specifications
ELISA: order BSA/sodium azide-free format for coating, Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
P21589
Host
Mouse
Reactivity
Human
Immunogen
A recombinant full length human NT5E protein was used as the immunogen for the CD73 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
NT5E/2646
Applications
ELISA, FACS, IF, WB, IHC-P
Purity
Protein G affinity chromatography
Format
Purified
Buffer
1 mg/ml in 1X PBS; BSA free, sodium azide free
Reconstitution
Store the CD73 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This CD73 antibody is available for research use only.
Storage Conditions
Store the CD73 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
1 mg/mL in 1X PBS; BSA free, sodium azide free
Location
Cell surface, cytoplasmic
Image Legend
Immunofluorescence staining of human U-87 MG cells with CD73 antibody (green, clone NT5E/2646) and Reddot nuclear stain (red) .

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