CD73 Antibody

• Description:

  CD73 is a membrane-bound extracellular enzyme overexpressed in several cancer types. Its expression has been linked to poor prognosis in melanoma, colorectal, gastric, triple negative breast cancer, and to a pro-metastatic phenotype in prostate cancer. Together with CD39, it plays a major role in promoting immunosuppression through the pathway degrading adenosine triphosphate (ATP) into adenosine. Within the tumor microenvironment, ATP promotes immune cell-mediated killing of cancer cells. In contrast, adenosine accumulation causes immune suppression, dysregulation of immune cell infiltrates and stimulates angiogenesis resulting in tumor spreading. CD73 is active on the last step of the degradation pathway, where it is the enzyme that actually degrades AMP into adenosine. CD73-blockade promotes anti-tumor immunity by reducing adenosine accumulation. Accordingly, anti-CD73 mAbs stimulate anti-tumor immunity and reduce tumor metastasis in mouse tumor models, and could enhance the efficacy of treatment with anti-PD1 or anti-CTLA4 antibodies.

• Specifications:

  ELISA: order Ab without BSA for coating, Flow cytometry: 2-4ug/million cells in 0.1ml, Immunofluorescence: 2-4 µg/mL, Western blot: 2-4 µg/mL, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 minutes at RT

• UniProt:

  P21589

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  Recombinant full-length human NT5E protein was used as the immunogen for the CD73 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG1 k

• Clone:

  NT5E/2646

• Applications:

  ELISA, FACS, IF, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Biotin Conjugate

• Buffer:

  0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution:

  Store the CD73 antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).

• Limitations:

  This CD73 antibody is available for research use only.

• Storage Conditions:

  Store the CD73 antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term) .

• Formulation:

  0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide

• Applications Notes:

  Optimal dilution of the CD73 antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cell surface

• Image Legend:

  IHC staining of FFPE human tonsil tissue with biotinylated CD73 antibody (clone NT5E/2646) . HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.