CD73 Antibody

CD73 Antibody

• Description: CD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.
• CAS Number: 9007-83-4
• UniProt: P21589
• Host: Mouse
• Immunogen: A recombinant full length human NT5E protein was used as the immunogen for the CD73 antibody.
• Clonality: Monoclonal
• Isotype: IgG1 κ
• Applications: ELISA, WB, FACS, IF, IHC-P
• Format: Purified
• Buffer: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Reconstitution: Store the CD73 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
• Limitations: This CD73 antibody is available for research use only.