MSH6 Antibody

• Description:

  The finding that mutations in DNA mismatch repair genes are associated with hereditary nonpolyposis colorectal cancer (HNPCC) has resulted in considerable interest in the understanding of the mechanism of DNA mismatch repair. Initially, inherited mutations in the MSH2 and MLH1 homologs of the bacterial DNA mismatch repair genes mutS and mutL were demonstrated at high frequency in HNPCC and were shown to be associated with microsatellite instability. A member of the mismatch repair family, GTBP (also designated MSH6), is an MSH2-related protein that binds to DNA containing G/T mismatches. Findings suggest that the mismatch-binding factor in human cells is composed of a heterodimer of GTBP and MSH2.

• Specifications:

  ELISA (order BSA/sodium azide-free format for coating), Flow cytometry: 1-2ug/10^6 cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT

• UniProt:

  P52701

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant human MSH6 protein fragment within amino acids 374-540 was used as the immunogen for the MSH6 antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  MSH6/3086

• Applications:

  ELISA, FACS, IF, WB, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide

• Reconstitution:

  Store the MSH6 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This MSH6 antibody is available for research use only.

• Storage Conditions:

  Store the MSH6 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number:

  9007-83-4

• Location:

  Nucleus

• Image Legend:

  Immunofluorescence staining of PFA-fixed human MCF-7 cells with MSH6 antibody (green, clone MSH6/3086) and Phalloidin (red) .