CD73 Antibody

• Description :

  The protein encoded by the NT5E gene is a plasma membrane protein that catalyzes the conversion of extracellular nucleotides to membrane-permeable nucleosides. The encoded protein is used as a determinant of lymphocyte differentiation. Defects in this gene can lead to the calcification of joints and arteries. Two transcript variants encoding different isoforms have been found for this gene. [RefSeq]

• Specifications :

  ELISA: order BSA-free format for coating, Flow cytometry: 1-2ug/million cells, Immunofluorescence: 1-2 µg/mL, Western blot: 1-2 µg/mL, Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 minutes at RT, Prediluted IHC only format: incubate for 30 min at RT (1)

• UniProt :

  P21589

• Host :

  Mouse

• Reactivity :

  Human

• Immunogen :

  A recombinant full length human NT5E protein was used as the immunogen for the CD73 antibody.

• Clonality :

  Monoclonal

• Isotype :

  IgG1 k

• Clone :

  CDLA73-1

• Applications :

  IHC-P, IF, WB, FACS, ELISA

• Purity :

  Protein G affinity chromatography

• Format :

  Purified

• Buffer :

  0.2 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide

• Reconstitution :

  Store the CD73 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations :

  This CD73 antibody is available for research use only.

• Storage Conditions :

  Store the CD73 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation :

  0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide

• CAS Number :

  9007-83-4

• Location :

  Cell surface, cytoplasmic

• Image Legend :

  Flow cytometry testing of human U-87 MG cells with CD73 antibody (clone CDLA73-1) ; Red=isotype control, Blue= CD73 antibody.