CD47 Antibody

CAT: 0800-V7535BTNSize: 500 µLDry Ice: NoHazardous: No
CAT#:0800-V7535BTNSize:500 µL
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PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This antibody reacts with Ig domain of CD47 protein. CD47, originally named integrin-associated protein (IAP), is a 50kDa protein containing five membrane-spanning sequences and a short cytoplasmic tail. CD47 plays a role in both cell adhesion by acting as an adhesion receptor for THBS1 on platelets, and in the modulation of integrins. It is important in memory formation and synaptic plasticity in the hippocampus. CD47 may play a role in membrane transport and/or integrin dependent signal transduction.
CAS Number
9007-83-4
Specifications
Immunofluorescence: 2-4 µg/mL, Flow cytometry: 5ul per million cells, Immunohistochemistry (FFPE) : 2-4 µg/mL for 30 minutes at RT
UniProt
Q08722
Host
Mouse
Reactivity
Human
Immunogen
A portion of amino acids 18-135 from the human protein was used as the immunogen for the CD47 antibody.
Clonality
Monoclonal
Isotype
IgG1 k
Clone
CD47/3019
Applications
IF, FACS, IHC-P
Purity
Protein G affinity chromatography
Format
Biotin Conjugate
Buffer
0.1 mg/ml with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
Reconstitution
Store the CD47 antibody at 2-8oC (up to one month) or aliquot and store at -20oC (longer term).
Limitations
This CD47 antibody is available for research use only.
Storage Conditions
Store the CD47 antibody at 2-8°C (up to one month) or aliquot and store at -20°C (longer term) .
Formulation
0.1 mg/mL with 0.1 mg/mL BSA (US sourced), 0.05% sodium azide
Applications Notes
Optimal dilution of the CD47 antibody should be determined by the researcher.
Location
Cell surface, cytoplasmic
Image Legend
IHC staining of FFPE human prostate carcinoma with biotinylated CD47 antibody (clone CD47/3019) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 10-20 min and allow to cool before testing.

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