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Recombinant MUC3 Antibody

CAT: 0800-V7512-20UGSize: 20 µgDry Ice: NoHazardous: No
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CAT#:0800-V7512-20UGSize:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
It recognizes a protein of HMW, identified as mucin 3 glycoprotein (MUC3) . Its epitope localizes between aa SITTTE. This MAb shows no cross-reaction with human milk fat globule membranes, MUC1, or MUC2. MUC3 is distributed in colon and rectum, and is also present to a lesser extent in breast, lung and salivary gland tissues. The Mucins are a family of highly glycosylated, secreted proteins with a basic structure consisting of a variable number of tandem repeats (VNTRs) encoded by 60 base pairs (Mucin 1), 69 base pairs (Mucin 2) and 51 base pairs (Mucin 3) . The number of repeats is highly polymorphic and varies among different alleles. Mucin 1 proteins are expressed as type I membrane proteins in addition to secreted forms. Mucin 1 is aberrantly expressed in epithelial tumors including breast carcinomas. Mucin 2 coats the epithelia of the intestines and airways and is associated with colonic tumors. Mucin 3 is a major component of various mucus gels and is broadly expressed in normal and tumor cells.
CAS Number
9000-83-3
Specifications
Immunohistochemistry (FFPE) : 1-2 µg/mL for 30 min at RT
UniProt
Q02505
Host
Rabbit
Reactivity
Human
Immunogen
Amino acids (C-HSTPSFTSSITTTETTSHSTPSFTSSITTTETTS) which contains two of the MUC3 tandem repeats was used as the immunogen for the MUC3 antibody.
Clonality
Recombinant Monoclonal
Isotype
IgG κ
Clone
MUC3/2992R
Type
Recombinant
Applications
IHC-P
Purity
Protein A affinity chromatography
Format
Purified
Buffer
0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
Reconstitution
Store the recombinant MUC3 antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).
Limitations
This recombinant MUC3 antibody is available for research use only.
Storage Conditions
Store the recombinant MUC3 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .
Formulation
0.2 mg/mL in 1X PBS with 0.1 mg/mL BSA (US sourced) and 0.05% sodium azide
Location
Cytoplasmic and cell surface
Image Legend
IHC staining of FFPE human gastric carcinoma with MUC3 antibody. HIER: boil tissue sections in 10mM Tris with 1mM EDTA, pH 9, for 10-20 min and allow to cool before testing.

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