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GAD2 / GAD65 antibody

CAT: 0013-GTR18398393-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398393-01Size:0.1 mL
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Description
GAD2 / GAD65antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
GAD65
UniProt
Q05329
Reactivity
Human
Immunogen
Recombinant human GAD2 (GAD65) protein fragment (around aa 6-99) (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-602M
Conjugation
Unconjugated
Field of Research
Neuroscience
Dilution
1:100-1:200
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Pancreas: A strong GAD2 staining should be seen in pancreatic islet cells. Negative Control: Colon: GAD2 immunostaining should be absent in all cell types Cellular Localization: Intracellular Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2b, kappa

UniProtKB · Q05329

Glutamate decarboxylase 2

DCE2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q05329
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GAD2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
4.1.1.15
Processing
—
Secondary accessions
Q9UD87
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Cellular component

Cell membraneCell projectionCytoplasmCytoplasmic vesicleGolgi apparatusMembraneSynapse

Molecular function

DecarboxylaseLyase

PTM

LipoproteinPalmitatePhosphoprotein

Biological process

Neurotransmitter biosynthesis

Ligand

Pyridoxal phosphate

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